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Thyroid Hormone Receptors - Methods and Protocols | Aria Baniahmad | Springer

Because thyroid hormone receptors TRs are important regulators of a large number of biological processes-ranging from brain development to control of vision in vertebrates-abnormal functioning of these receptors can have serious medical consequences. In Thyroid Hormone Receptors: Methods and Protocols, a panel of outstanding investigators surveys and explains the major cutting-edge methods used in TR research and their practical experimental details. Described in step-by-step detail to ensure robust experimental results, the techniques presented cover a wide variety of key areas, including TRs in development and generation of knockout mouse and Xenopus , transcriptional regulation by TRs in both cell-free systems and living cells, and TR mutant analysis of patients.

Additional methods provide powerful tools for the isolation of TR-regulated protein complexes, for the study of blood cell differentiation through the oncogene v-erbA, and for target gene analysis in the brain. Microarray chip methods are also presented for analyzing the organs of transgenic mice to identify target genes in the liver. Each protocol has been developed by a hands-on expert and contains full explanatory notes, troubleshooting tips, and helpful comments on avoiding pitfalls.

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Broad ranging and highly practical, Thyroid Hormone Receptors: Methods and Protocols offers endocrinologists, geneticists, biochemists, and research physicians a comprehensive collection of powerful methods useful not only for all major areas of TR biology and analysis, but also for nuclear hormone receptors in general. JavaScript is currently disabled, this site works much better if you enable JavaScript in your browser.

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Download Datasheet. Download CofA:. Thyroid Hormone Receptor beta 1 Antibody Description. The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone.

Defects in this gene are known to be a cause of generalized thyroid hormone resistance GTHR , a syndrome characterized by goiter and high levels of circulating thyroid hormone T3-T4 , with normal or slightly elevated thyroid stimulating hormone TSH. This TR Beta1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human THRB isoform 1 protein. Immunogen Type. Storage Condition. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial.

Mechanism of action of Thyroxine ( synthesis of thyroid hormones ) Physiology animations

Use this intermediate dilution when calculating final dilutions as recommended below. Avoid cycles of freezing and thawing. Application Note. Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human THRB protein. Cross-reactivity with THRB from other sources has not been determined.

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Disclaimer Note-General. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture USDA inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation.

All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale.

Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc.

If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc. General Reference. Cheng, Sheue-yann. Specific Reference.


J Mol Endocrinol. Epub Jan PLoS One. Print View product citations for antibody A96S on CiteAb. Log in to submit your product review. Product Type. Reacts With. Catalog Number. Conjugation Reference. Molecular Weight.